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Q from U: Is thawing and refreezing embryos harmful to the embryo? I think this would make a great discussion topic. In a perfect world we’d like to do a complete chromosomal screening on them to find which ones are euploid. My RE says this is possible and the embryologist would thaw, biopsy and re-vitrify them.

She states the lab has had a lot if success doing this. My concern is for the damage to the embryos. How good are labs at doing this? In my own lab in Indy, we found ourselves  in the position of having to refreeze when we thawed more than we needed because we assumed fewer embryos would survive. Live birth of a normal healthy baby after a frozen embryo transfer with blastocysts that were frozen and thawed twice. This is a report on the birth of a normal, healthy baby after embryo transfer using blastocysts that have been twice frozen and twice thawed. Eight frozen blastocysts were thawed for a frozen embryo transfer.

One blastocyst was transferred to the uterus and four of the thawed blastocysts were refrozen. The patient became pregnant but suffered a miscarriage at 7 weeks. The four remaining blastocysts were re-thawed and all four transferred. Here’s a much larger study that looked at the same question in 49 IVF cases. The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method. This retrospective study in a private fertility clinic  looked at outcomes from 49 women who had excess embryos refrozen. These women had embryos thawed for a frozen embryo transfer cycle, some of which remained as excess embryos. The embryos remaining after the transfer were refrozen and re-thawed in a future frozen embryo transfer cycle.

I loved reading all of your stories. Silber and staff for bringing our beauitful baby girl into this world. I think that’s what makes it unique. Advertising revenue supports our not-for-profit mission. Keep up the good work on the weight loss journey.

It’s clear from the way Matt talks that he loves his wife and wants nothing more than to be a dad. Don’t know how to comprehend the status. After 3 embryo transfer I’m on 600mg Utrogestan per day, orally, and a 25mg shot of Prolutex in the morning. In fact, the acid concentration inside the embryo is normally much greater than that, and the oxygen concentration is much lower. Was told at the start to use donor eggs but was advised to give IVF a shot.

Assisted reproductive technology: an overview of Cochrane Reviews”. Before we just found it easier to avoid, because finding out another person is pregnant was heart breaking. It felt so weird when I came back not to be able to acknowledge that I had gone through this huge loss. My husband had a vasectomy during his first marriage and when we married, a failed reversal so IVF is our only option. Thank you for your beautifully worded response.

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The pregnancy rates and implantation rates between those embryo that had undergone one freeze-thaw versus two freeze-thaw cycles were compared. Does biopsying the embryos before refreezing make the embryos less able to survive -quality intact- through a second round of refreezing? Refreezing of murine intact and biopsied embryos by rapid-freezing procedure. The advantage of mouse studies is that you can collect a lot of data in a short period of time, This study looked at how 860 mouse embryos fared after freezing , thawing with or without biopsy, then refreezing and re-thawing. They looked at the implantation and pregnancy rates of mice who had a transfer with these embryos.

With the advent of genetic screening of biopsied embryos, which based on the last ASRM meeting, is becoming more and more widespread in US clinics, the freezing of blastocysts is becoming routine after biopsy and results are good with thawing of these once-frozen blastocysts. However, as with every intervention in the IVF lab, it can be done poorly or well. Keep in mind, for this to work, freezing, thawing and biopsy must be done with a high level of skill multiple times on the embryo. Highly skilled ART techs can do this. What data do you have on outcomes from this approach? Implantation rate, pregnancy rate and live birth rate.

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The reader who asked this question shared that her RE told them they have a lot of success with this. Then they shouldn’t mind sharing numbers. If you are the first patient for any new intervention, you have no information to go on and your risk of a poor outcome is not calculable. The key is getting enough information as possible about risks, benefits and alternatives before you agree to any medical intervention. I have 2 follow up questions, if you don’t mind. Does it matter if they’re day 6 blasts? Almost all technology gets better with time and I would imagine that there are a lot of couple in this type of position.

I know some labs an get PGD results in 24 hours. Do you see the technology progressing enough that the results of the PGD could be done in few enough hours to make a fresh transfer possible? Thank you for everything you do! Day 6 blasts are in a sense living on borrowed time. The pregnancy rate from day 6 blasts is lower than day 5 blasts. If I had a choice, I would prefer to do the biopsy on day 5 blasts, either fresh or thawed.

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